t-Boc-N-amido-PEG3-propargyl is a crosslinker containing a propargyl group and a t-butyloxycarbonyl (t-Boc) protected amine. Its molecular formula is C₁₄H₂₅NO₅, with a molecular weight of 287.35. The structure consists of a tri(ethylene glycol) (PEG3) chain linking the propargyl group to a primary amine group protected by the t-Boc moiety. The propargyl group can participate in copper-catalyzed click chemistry reactions, while the t-Boc group undergoes deprotection under weakly acidic conditions to release free amine. The production process requires precise control of reaction conditions to achieve high purity (≥97%). This reagent is widely used in bioconjugation, protein imaging, and materials science, such as enabling specific protein modification via click chemistry or preparing dynamic hydrogels, making it an important tool in biomedical research.