Bromo-PEG3-Bromide With CAS.31255-26-2 Is For Chmical Modifications.

Introduction

Bromo-PEG3-Bromide With CAS.31255-26-2 is a monodentate coboxyl-terminated polyethylene glycol (PEG)-containing reagents with either a methyl ether, hydroxyl, azido, or triplebonds. It has defined molecular weights and spacer lengths and are used for modifying Proteins or surfaces witch containing amine groups such quantum dots, self-assembled monolayers and magnetic particles. Functionalization of solid surfaces with polyethylene glycol spacers significantly reduces nonspecific protein binding.

Important Product Information

? Bromo-PEG3-Bromide With CAS.31255-26-2 is low-melting solids that are difficult to weigh and dispense. To facilitate handling, make a stock solution by dissolving the reagent in dimethylsulfoxide (DMSO) or dimethylformamide (DMF).

? Store unused stock solution at -20°C. Equilibrate reagent vial to room temperature before opening to avoid moisture condensation. To minimize air exposure, keep the stock solution under an inert gas such as argon or nitrogen. Cap the stock solution with a septum and use a syringe to remove the solution.

? If the reagent is used for surface binding and further protein loading, the reagent-to-surface ratio in the reaction affect the number of carboxylic acid residues available for further modification. Optimize these ratios to obtain the modification level needed for the specific application.

? For Use non - amine - containing buffers at pH 7-9 such as PBS (20mM sodium phosphate, 150mM NaCl; pH 7.4); 100mM carbonate/biocarbonate; or 50mM borate. Do not use buffers that contain primary amines, such as Tris or glycine, which compete with acylation.